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Phenotypic methods of identifying microorganisms in a patient sample are characterized as those that ________.


A) directly examine the organism's appearance or behavior, which includes its metabolic abilities,environmental preferences and drug susceptibilities
B) analyze the genetic makeup of the microorganism,which conclusively diagnoses the infection
C) make use of the patient's antibodies to precipitate the microorganism out of solution,or agglutinate the antigens in the sample
D) make use of color-changing antibodies directed against the microbe that will become visible to the naked eye when bound

E) B) and D)
F) A) and C)

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Titer is the amount of ________.


A) antigen in serum
B) antibody in serum
C) WBC in serum
D) complement in serum
E) memory cells in serum

F) A) and D)
G) C) and E)

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Which sample is not typically collected by sterile needle aspiration?


A) Blood
B) Urine
C) Cerebrospinal fluid
D) Tissue fluids
E) All of the choices are collected by sterile needle aspiration.

F) C) and D)
G) All of the above

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Which of the following is not one of the main categories for identifying microbes?


A) Radiologic
B) Phenotypic
C) Genotypic
D) Immunologic

E) All of the above
F) B) and C)

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Specimen collection ________.


A) is always done by a medical professional
B) must be done under sterile conditions
C) must utilize aseptic techniques
D) does not require special handling
E) All of the choices are correct.

F) A) and B)
G) A) and C)

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Techniques that make use of probes that hybridize to specific sequences of a pathogen's genome include ________.


A) FISH and microarrays
B) ELISA and FISH
C) FISH and Western blot
D) PFGE and ELISA

E) A) and D)
F) A) and C)

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A student nurse on a medical-surgical floor is caring for a 62-year-old previously healthy female. The patient has been admitted for an infectious disease work-up. She has been experiencing generalized pain and nausea for the past three weeks, and recently developed a fever. The student nurse prepares to assist the RN in collecting blood for the following lab tests: complete blood count (CBC) , C-reactive protein (CRP) , eosinophil sedimentation rate (ESR) , blood culture, urine culture, and a lumbar puncture with cerebral spinal fluid (CSF) culture and cell count. The nurse is also collecting a sputum culture, as well as a respiratory PCR by nasopharyngeal suction. -The lumbar puncture was especially uncomfortable for the patient.What step listed below is most important to be taken to ensure the procedure does not need to be repeated?  


A) The specimens must be properly labeled.
B) The specimens must be properly obtained and stored.
C) The specimens must be collected in sufficient amounts.
D) The specimens must be properly transported.
E) All of the choices are equally important.

F) B) and E)
G) All of the above

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The public health department is following a 34-year-old male who has reported several weeks of general malaise and a low-grade fever. The nurse assigned to his case communicates with the patient regarding the results of his laboratory testing. -An ELISA test sent for HIV screening came back with a positive result.The nurse educates the patient that this test is highly sensitive with low specificity. Which of the following statements by the patient demonstrates understanding of this teaching?


A) It is possible that this is a false-positive result since the test may have been positive for something other than HIV.
B) It is unlikely that this is a false-positive result since the test is so specific it does not miss a positive result.
C) It is possible that this is a false-positive result since low specificity means the tests are less accurate.
D) It is unlikely that this is a false-positive result since it is highly sensitive.

E) B) and D)
F) All of the above

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An outbreak of E.coli 0157:H7 was suspected to be associated with the petting zoo enclosure at the state fair.  Epidemiologists with the CDC created a DNA fingerprint from the E.coli isolated from patients and compared it to bacterial isolates analyzed by national laboratories.  They confirmed that the organism was indeed being transmitted by the animals at the fair.  The CDC uses what method to generate these fingerprints?


A) PFGE
B) FISH
C) Microarray
D) Hybridization

E) All of the above
F) B) and D)

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The Weil-Felix test is used for diagnosing salmonellosis.

A) True
B) False

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Antibody testing requires ________.


A) a known antigen
B) a known antibody
C) both a known antigen and a known antibody
D) either a known antigen or a known antibody

E) A) and B)
F) A) and C)

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MRI,CT and PET scans are useful diagnostic tools in cases where infection is located in deep tissues,thereby saving the patient from an invasive biopsy.

A) True
B) False

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Biochemical tests include all of the following except ________.


A) presence of catalase
B) presence of oxidase
C) colony morphology
D) sugar fermentation
E) gas production

F) A) and D)
G) A) and C)

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Which of the following is not a phenotypic method of identification?


A) Morphology
B) Gram stain reaction
C) Acid-fast reaction
D) Antibody response
E) Endospores

F) B) and D)
G) All of the above

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Serological tests should have low sensitivity and specificity.

A) True
B) False

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Each of the following are appropriate specimens for bacterial culture except ________.


A) saliva
B) skin
C) spinal fluid
D) hair
E) throat

F) B) and D)
G) B) and C)

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The public health department is following a 34-year-old male who has reported several weeks of general malaise and a low-grade fever. The nurse assigned to his case communicates with the patient regarding the results of his laboratory testing. -The patient reports that he had a negative HIV PCR test about a month ago.What is the most appropriate interpretation of this information by the nurse? 


A) PCRs are rarely accurate.
B) The ELISA was a false-positive.
C) It is possible the ELISA was a false-positive,and the Western blot must be performed to confirm the results.
D) The HIV PCR is outdated and does not pick up modern strains of the virus.

E) None of the above
F) A) and B)

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The value of whole genome sequencing is that it can separate larger strands of DNA by slowly applying alternating voltage levels from three different directions.

A) True
B) False

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A student nurse on a medical-surgical floor is caring for a 62-year-old previously healthy female. The patient has been admitted for an infectious disease work-up. She has been experiencing generalized pain and nausea for the past three weeks, and recently developed a fever. The student nurse prepares to assist the RN in collecting blood for the following lab tests: complete blood count (CBC) , C-reactive protein (CRP) , eosinophil sedimentation rate (ESR) , blood culture, urine culture, and a lumbar puncture with cerebral spinal fluid (CSF) culture and cell count. The nurse is also collecting a sputum culture, as well as a respiratory PCR by nasopharyngeal suction. -The patient's respiratory PCR results are reported as "insufficient cells available for testing." Which of the following statements by the student nurse indicates proper understanding of the findings? 


A) The patient is free of infection since insufficient cells could be isolated.
B) A smaller sample must be sent,since the irrigation solution volume diluted the cells.
C) This is a very specific and sensitive finding that points to a viral infection.
D) The sample was inadequate,and the nasopharyngeal specimen collection must be repeated.

E) None of the above
F) C) and D)

Correct Answer

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A student nurse on a medical-surgical floor is caring for a 62-year-old previously healthy female. The patient has been admitted for an infectious disease work-up. She has been experiencing generalized pain and nausea for the past three weeks, and recently developed a fever. The student nurse prepares to assist the RN in collecting blood for the following lab tests: complete blood count (CBC) , C-reactive protein (CRP) , eosinophil sedimentation rate (ESR) , blood culture, urine culture, and a lumbar puncture with cerebral spinal fluid (CSF) culture and cell count. The nurse is also collecting a sputum culture, as well as a respiratory PCR by nasopharyngeal suction. -The patient's CRP is markedly elevated.What does this indicate? 


A) An elevated CRP is a highly sensitive urinary tract infection marker.
B) An elevated CRP points to inflammation from any of a variety of sources.
C) An elevated CRP is diagnostic of a fungal infection.
D) An elevated CRP is diagnostic of a bacterial infection.

E) C) and D)
F) None of the above

Correct Answer

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