Question 1

Which of the following is a promising treatment for stopping the expression of an unwanted gene?

Accepted Answer

A)   Antisense therapy
B)   DNA ﬁngerprinting
C)   Cloning
D)   Gene therapyE) None of the above
F) C) and D)

Question 2

A proteome is an organism's full array of expressed proteins, and proteomics is the study of thoseproteins and the functions they mediate.

Accepted Answer

A) True 
 B)False

Question 3

EcoRI and HindIII are ______.

Accepted Answer

A)   palindromes
B)   reverse transcriptases
C)   restriction endonucleases
D)   ligases
E)   DNA polymerasesF) D) and E)
G) None of the above C

Question 4

Identi?cation of unique DNA ?ngerprints relies on the presence of single nucleotide polymorphisms among samples.

Accepted Answer

A) True 
 B)False

Question 5

Analysis of DNA fragments in gel electrophoresis is based on ______.

Accepted Answer

A)   larger fragments moving slowly and remaining closer to the wells
B)   DNA having an overall negative charge and moving to the positive pole
C)   DNA fragments being stained so that they can be seen
D)   application of an electric current through the gel causing DNA fragments to migrate
E)   All of the choices are correct.F) A) and D)
G) A) and C)

Question 6

The ﬁrst genetically engineered protein approved for human use was ______.

Accepted Answer

A)   hemophilia factor VIII
B)   human insulin
C)   human growth hormone
D)   human adrenaline
E)   human testosteroneF) All of the above
G) A) and D)

Question 7

Transgenic animals ______.

Accepted Answer

A)   can be engineered to become factories for manufacturing proteins
B)   are often obtained from germline engineering
C)   will pass the genes on to their offspring
D)   commonly include mice
E)   All of the choices are correctF) B) and E)
G) B) and D)

Question 8

Recombinant strains of _____ are released to colonize plant roots to produce an insecticide to destroy invading insects.

Accepted Answer

A)   Pseudomonas ?uorescens
B)   Saccharomyces cerevisiae
C)   Pseudomonas syringae
D)   Escherichia coli
E)   Thermus aquaticusF) A) and B)
G) B) and E)

Question 9

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants, thereby reducing freezing of plants and ?nancial distress to the farmers as a result of freezing weather. This product contains a strain of ______.

Accepted Answer

A)   Escherichia coli
B)   Pseudomonas syringae
C)   Thermus aquaticus
D)   Saccharomyces cerevisiae
E)   Pseudomonas ?uorescensF) D) and E)
G) A) and E) B

Question 10

One reason the ﬁeld of proteomics is continually evolving following the systematic study of an organism's genome is because ______.

Accepted Answer

A)   it became apparent that while the organism's genome is relatively stable, protein expression in an organism is constantly changing
B)   it became apparent that protein expression is relatively uniform, whereas the genome is constantly changing
C)   once the genome had been sequenced, all protein expression was understood
D)   the expression of proteins is ﬁxed in each organism so sequencing the genome is essential to understanding phenotypesE) None of the above
F) C) and D)

Question 11

Ampliﬁcation of DNA is accomplished by ______.

Accepted Answer

A)   a Western blot
B)   DNA sequencing
C)   a Southern blot
D)   the polymerase chain reaction
E)   gene probesF) A) and B)
G) C) and E)

Question 12

Which of the following techniques is mismatched with its use in DNA ﬁngerprinting?

Accepted Answer

A)   PCR ampliﬁcation to get more copies of DNA
B)   Southern blot for a visual record of DNA fragments
C)   Hybridization probes to digest the DNA sample
D)   Restriction endonucleases to cut DNA
E)   Electrophoresis to separate DNA fragmentsF) A) and B)
G) A) and C)

Question 13

Which of the following is not true of ﬂuorescent in situ hybridization (FISH) ?

Accepted Answer

A)   Probes can be applied to intact cells.
B)   Probes can locate genes on chromosomes.
C)   It can be used to identify unknown bacteria without culturing.
D)   It uses electrophoresis to separate the DNA.
E)   It can be used to detect RNA in cells.F) A) and E)
G) C) and E)

Question 14

Transgenic animals are referred to as _____ modi?ed organisms.

Accepted Answer

A)   naturally
B)   genetically
C)   physically
D)   chemicallyE) A) and B)
F) C) and D)

Question 15

Which PCR step synthesizes complimentary DNA strands?

Accepted Answer

A)   Cool DNA to between 50° C and 65° C.
B)   Add DNA polymerase and nucleotides at 72° C.
C)   Add primers.
D)   Heat target DNA to 94° C.E) A) and D)
F) All of the above

Question 16

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are ______.

Accepted Answer

A)   sources of heat-stable DNA polymerases
B)   genetically engineered bacteria
C)   used as cloning vectors
D)   principal sources of restriction endonucleasesE) C) and D)
F) All of the above A

Question 17

The advantage of germline therapy over somatic cell therapy is that the normal gene is inserted into an egg, sperm, or developing embryo so that the repair is present in every cell of the organism as it matures to adulthood.

Accepted Answer

A) True 
 B)False

Question 18

Which of the following is the second step in gene cloning?

Accepted Answer

A)   Target DNA removed from cells and isolated
B)   Cloning host treated with calcium chloride and receives plasmid
C)   Separate DNA fragments with gel electrophoresis
D)   Desired protein is produced by cloning host
E)   Gene is ampli?ed by multiplication of cloning hostF) A) and E)
G) B) and C)

Question 19

Before the advent of biotechnology, diabetics would use insulin harvested from cows. Now they receive human insulin produced through ______.

Accepted Answer

A)   gene therapy
B)   PCR
C)   gel electrophoresis
D)   recombinant DNA technologyE) B) and C)
F) All of the above

Question 20

The ?eld of pharmacogenomics uses knowledge of an individual's single nucleotide polymorphisms to determine how they will respond to a particular drug.

Accepted Answer

A) True 
 B)False

Exam 10: Genetic Analysis and Genetic Engineering

Which of the following is a promising treatment for stopping the expression of an unwanted gene?

Correct AnswerverifiedShow Answer

A proteome is an organism's full array of expressed proteins, and proteomics is the study of thoseproteins and the functions they mediate.

Correct AnswerverifiedShow Answer

EcoRI and HindIII are ______.

Correct AnswerverifiedC

Identi?cation of unique DNA ?ngerprints relies on the presence of single nucleotide polymorphisms among samples.

Correct AnswerverifiedShow Answer

Analysis of DNA fragments in gel electrophoresis is based on ______.

Correct AnswerverifiedShow Answer

The ﬁrst genetically engineered protein approved for human use was ______.

Correct AnswerverifiedShow Answer

Transgenic animals ______.

Correct AnswerverifiedShow Answer

Recombinant strains of _____ are released to colonize plant roots to produce an insecticide to destroy invading insects.

Correct AnswerverifiedShow Answer

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants, thereby reducing freezing of plants and ?nancial distress to the farmers as a result of freezing weather. This product contains a strain of ______.

Correct AnswerverifiedB

One reason the ﬁeld of proteomics is continually evolving following the systematic study of an organism's genome is because ______.

Correct AnswerverifiedShow Answer

Ampliﬁcation of DNA is accomplished by ______.

Correct AnswerverifiedShow Answer

Which of the following techniques is mismatched with its use in DNA ﬁngerprinting?

Correct AnswerverifiedShow Answer

Which of the following is not true of ﬂuorescent in situ hybridization (FISH) ?

Correct AnswerverifiedShow Answer

Transgenic animals are referred to as _____ modi?ed organisms.

Correct AnswerverifiedShow Answer

Which PCR step synthesizes complimentary DNA strands?

Correct AnswerverifiedShow Answer

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are ______.

Correct AnswerverifiedA

The advantage of germline therapy over somatic cell therapy is that the normal gene is inserted into an egg, sperm, or developing embryo so that the repair is present in every cell of the organism as it matures to adulthood.

Correct AnswerverifiedShow Answer

Which of the following is the second step in gene cloning?

Correct AnswerverifiedShow Answer

Before the advent of biotechnology, diabetics would use insulin harvested from cows. Now they receive human insulin produced through ______.

Correct AnswerverifiedShow Answer

The ?eld of pharmacogenomics uses knowledge of an individual's single nucleotide polymorphisms to determine how they will respond to a particular drug.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
C

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
B

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
A

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified