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The polymerase chain reaction uses RNA polymerase.

A) True
B) False

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State the difference between a transgenic animal and a cloned animal.

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Transgenic organisms are also called gen...

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Explain the applications for polymerase chain reaction technology.

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Answers will vary but should include the...

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Transgenic ________ have been given suicide genes that cause them to self-destruct when the job for which they are engineered has been accomplished.


A) animals
B) bacteria
C) plants
D) fungi
E) All of the choices are correct.

F) A) and C)
G) C) and E)

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Polymerase chain reactions utilize a heat-stable DNA polymerase that was isolated from the bacterium Thermus aquaticus.

A) True
B) False

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Small,variable regions of DNA that may change an individual's susceptibility to disease are called SNPs (single nucleotide polymorphisms).

A) True
B) False

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Gene therapy is being used in cancer patients to make healthy cells more tolerant of chemotherapy and to make tumors more vulnerable to chemotherapy.

A) True
B) False

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Gene pharming is the use of


A) transgenic plants to produce larger vegetables and sweeter fruits.
B) bacteria for bioremediation.
C) STR profiling to determine evolutionary relationships among organisms.
D) transgenic farm animals to produce pharmaceuticals.

E) None of the above
F) C) and D)

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Gene cloning can be accomplished by recombinant DNA technology and polymerase chain reactions.

A) True
B) False

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Plants have been genetically engineered for all of the following reasons EXCEPT


A) to have a higher vitamin A content.
B) to make the plant more resistant to pests.
C) to produce human proteins.
D) to reduce food spoilage.
E) All of the choices are correct.

F) D) and E)
G) C) and E)

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All of the following are true regarding transgenic animals EXCEPT


A) foreign genes are micro-injected into eggs,fertilized in vitro,and the zygotes are placed in host females to develop.
B) the process has created mice that have a phenotype similar to that of a person with cystic fibrosis.
C) the process has been used to develop organisms that are partially animal and partially plant.
D) the product desired by the transgenic procedure may be secreted in the milk of female offspring.

E) A) and B)
F) None of the above

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The function of DNA ligase in recombinant technology is to


A) cut DNA into many fragments.
B) carry DNA into a new cell.
C) seal DNA into an opening created by restriction enzymes.
D) make millions of copies of a specific segment of DNA.
E) separate fragments of DNA by their length and electrical charges.

F) B) and D)
G) A) and B)

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Familial hypercholesterolemia


A) is characterized by high blood levels of cholesterol.
B) is a condition that develops when liver cells lack a receptor protein for removing cholesterol from the blood.
C) may be treated by ex vivo gene therapy.
D) All of the answer choices are true.
E) Only two of the answer choices are true.

F) A) and C)
G) C) and D)

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Transgenic plant products include all of the following EXCEPT


A) soybeans that are resistant to a common herbicide.
B) an antibody produced by soybeans to treat genital herpes.
C) suicide genes that cause the plants to self-destruct after their product has been produced.
D) antibodies produced by corn plants that deliver radioisotopes to tumor cells.

E) B) and C)
F) A) and D)

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To carry out a polymerase chain reaction (PCR) ,you must have DNA polymerase and


A) a blueprint or gene map of the sequence you wish to copy.
B) the nucleotides to synthesize new DNA strands.
C) a DNA synthesizer machine.
D) a DNA probe.

E) None of the above
F) B) and D)

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PCR is a relatively new advance in DNA technology that targets specific sequences in the DNA for removal or replacement.

A) True
B) False

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Leber congenital amaurosis (LCA) is a disorder characterized by early-onset retinal dystrophy which causes severe visual impairment beginning in infancy.The most common cause of the disorder is a mutated intron located in gene CEP290.Targeted deletion of the mutated intron shows promise as a treatment for LCA.The treatment of LCA is an example of the application of which of the following types of biotechnology?


A) PCR
B) cloning
C) recombinant DNA technology
D) microarrays
E) genome editing

F) B) and D)
G) C) and E)

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What is the first step in producing a transgenic animal?


A) implantation of a donor egg into the host mother
B) inject the desired gene(s) into a donor egg
C) transgenic organism produces offspring that have the desired gene which can then produce more transgenic offspring
D) transgenic eggs are harvested from the surrogate mother

E) All of the above
F) B) and C)

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The introduction of normal genes into an afflicted individual for therapeutic use is called


A) human cloning.
B) proteomics.
C) gene therapy.
D) genetic profiling.

E) C) and D)
F) B) and C)

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In what field of study would scientists predict a protein's three-dimensional shape and how DNA mutations would affect protein function?


A) bioinformatics
B) proteomics
C) genomics
D) All of the answer choices.

E) All of the above
F) B) and C)

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