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Gram-negative bacteria do not have peptidoglycan in their cell walls.

A) True
B) False

Correct Answer

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False

The difference in cell wall structure of Mycobacterium and Nocardia compared to the typical gram-positive bacterial cell wall structure is that they ________.


A) contain more peptidoglycan
B) have a predominance of unique, waxy lipids
C) are easily decolorized
D) contain lipopolysaccharide
E) All of the choices are correct.

F) C) and E)
G) A) and E)

Correct Answer

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A client has a serious case of the flu. A random sample of sputum was taken from the patient coughing up blood. The lab tech said they had isolated a bacterium that did not have any peptidoglycan. You hypothesize that the identity of this microbe could possibly be ________.


A) Mycobacterium tuberculosis
B) Borrelia burghdorferi
C) Streptococcus pneumoniae
D) Mycoplasma pneumoniae
E) Staphylococcus aureus

F) None of the above
G) A) and B)

Correct Answer

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Iodine is the decolorizer in the Gram stain.

A) True
B) False

Correct Answer

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False

Which of the following is mismatched?


A) Gracilicutes - gram-negative cell walls
B) Firmicutes - gram-positive cell walls
C) Tenericutes - waxy acid-fast cell walls
D) Mendosicutes - archaea cell walls
E) None of the choices are correct.

F) A) and E)
G) A) and D)

Correct Answer

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A flagellum is anchored into the bacterial cell envelope by its ________.


A) hook
B) outer membrane
C) filament
D) sheath
E) basal body

F) All of the above
G) B) and E)

Correct Answer

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The enzyme ________, found in tears and saliva, can hydrolyze the bonds in the glycan chains of certain bacterial cell walls.


A) penicillinase
B) lysozyme
C) peptidase
D) All of the choices are correct.
E) None of the choices are correct.

F) A) and E)
G) D) and E)

Correct Answer

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The laboratory report concludes that the diagnosis is salmonellosis caused by Salmonella infection. Remembering resources available for prokaryotic identification, the RN speculates that the laboratory technicians may have used


A) Bergey's Manual of Systematic Bacteriology because rRNA analysis was required in the identification of this pathogen.
B) Bergey's Manual of Systematic Bacteriology because its five volumes of information were necessary for phenotypic identification of this pathogen.
C) Bergey's Manual of Determinative Bacteriology because they needed to fully understand the evolutionary background of the pathogen.
D) Bergey's Manual of Determinative Bacteriology because it classifies prokaryotes based on phenotypic traits typically tested for in clinical settings.

E) A) and D)
F) A) and C)

Correct Answer

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Which of the following is not a phenotypic trait of bacteria?


A) rRNA sequencing
B) Shape
C) Growth pattern in a culture medium
D) Biochemical reaction
E) All of these are phenotypic traits.

F) B) and E)
G) B) and D)

Correct Answer

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Boiling water (100°C) can normally destroy endospores.

A) True
B) False

Correct Answer

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What three components comprise the flagellum, extending from the cytoplasm to the exterior of the cell?


A) Filament, hook, basal body
B) Filament, basal body, hook
C) Basal body, hook, filament
D) Hook, basal body, filament
E) Basal body, filament, hook

F) B) and C)
G) C) and E)

Correct Answer

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A bacterial genus that has waxy mycolic acid in the cell walls is ________.


A) Mycobacterium
B) Mycoplasma
C) Streptococcus
D) Corynebacterium
E) Salmonella

F) C) and D)
G) B) and C)

Correct Answer

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If you observe rod-shaped, pink cells on a slide that had just been Gram-stained, you can assume that their cell walls contain endotoxin.

A) True
B) False

Correct Answer

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Cellular organisms without a true nucleus include bacteria and viruses.

A) True
B) False

Correct Answer

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Which of the following statements is not true regarding the differences between bacteria, archaea, and eukaryotes?


A) The cell walls of bacteria and archaea both contain the complex macromolecule peptidoglycan, whereas only the organisms in kingdom Plantae contain cell walls, which are made of cellulose.
B) Bacteria and archaea have circular chromosomes in a nucleoid region whereas eukaryotes have linear chromosomes that are enclosed in a nuclear membrane.
C) Flagella are found on bacteria, archaea and eukaryotes yet they all vary in structure.
D) Ribosomal RNA sequencing revealed that the structure of the ribosomal subunits in archaea more closely resemble those of eukaryotes than bacteria.

E) None of the above
F) A) and B)

Correct Answer

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Archaea do not have the typical peptidoglycan structure found in bacterial cell walls.

A) True
B) False

Correct Answer

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True

Specimen collection from the patient is ordered for microbial analysis in the hospital laboratory. Based upon the patient's symptoms, which of the following samples would the RN expect to be ordered for collection?


A) Aseptic collection of cerebrospinal fluid
B) Clean catch urine sample
C) Stool sample
D) Aseptic collection of sputum

E) A) and D)
F) A) and C)

Correct Answer

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Gram staining reveals the presence of gram-negative bacilli in the patient's stool. The RN understands how this bacterium would cause the patient's spike in temperature. Which of the following is the correct explanation for this patient's fever?


A) Gram-negative bacteria have a thick peptidoglycan layer which confers their ability to cause fever.
B) Gram-negative bacteria have two periplasmic spaces, which enhance their ability to cause fever.
C) Gram-negative bacteria have porin proteins in their outer membrane, which confer their ability to cause fever.
D) Gram-negative bacteria have lipopolysaccharide in their outer membrane, and the lipid portion confers their ability to cause fever.

E) B) and D)
F) C) and D)

Correct Answer

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A bacterial cell wall that has primarily peptidoglycan with small amounts of teichoic acid and lipoteichoic acid is ________.


A) gram-negative
B) gram-positive
C) found in archaea
D) a spheroplast
E) acid fast

F) B) and D)
G) B) and C)

Correct Answer

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The cell envelope or its parts can interact with human tissue and cause disease.

A) True
B) False

Correct Answer

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