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What type of test will detect whole antigens?


A) Cross reaction
B) Agglutination
C) Precipitation
D) Specificity
E) Sensitivity

F) B) and E)
G) A) and B)

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The Western blot test is confirmatory for HIV because ________.


A) it is more sensitive than the ELISA
B) it has fewer false positives than the ELISA
C) it tests for more HIV antibodies than ELISA
D) it is easier to interpret than ELISA
E) All of the choices are correct.

F) None of the above
G) A) and C)

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A positive indirect ELISA result requires ________.


A) one known antibody, one unknown antibody, and an unknown antigen
B) a known antigen, an unknown antibody, and a known antibody
C) two known antibodies and one known antigen
D) two known antibodies and one unknown antigen
E) All of the choices are correct.

F) C) and D)
G) D) and E)

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Serological tests should have low sensitivity and specificity.

A) True
B) False

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The tuberculin test is an example of an in vitro serological test.

A) True
B) False

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False

The Weil-Felix test is used for diagnosing salmonellosis.

A) True
B) False

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The patient reports that he had a negative HIV PCR test about a month ago. What is the most appropriate interpretation of this information by the nurse?


A) PCRs are rarely accurate.
B) The ELISA was a false-positive.
C) It is possible the ELISA was a false-positive, and the Western blot must be performed to confirm the results.
D) The HIV PCR is outdated and does not pick up modern strains of the virus.

E) None of the above
F) A) and B)

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Biochemical tests include all of the following except ________.


A) presence of catalase
B) presence of oxidase
C) colony morphology
D) sugar fermentation
E) gas production

F) B) and D)
G) A) and D)

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Soluble antigens are detected in this type of test.


A) Cross reactions
B) Agglutination
C) Precipitation
D) Specificity
E) Sensitivity

F) A) and C)
G) B) and E)

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Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test for HIV?


A) Immunochromatography
B) Western blot
C) Immunelectrophoresis
D) Radioimmunoassay (RIA)
E) Weil-Felix

F) B) and E)
G) A) and B)

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B

When minute samples of DNA need to be genetically analyzed for identification purposes, which test can be effectively used?


A) Direct fluorescence antibody
B) Immunofluorescence
C) Weil-Felix reaction
D) PCR
E) Direct antigen reaction

F) A) and C)
G) A) and E)

Correct Answer

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Immunologic methods of identifying microorganisms in a patient sample are characterized as those that ________.


A) rely on the specificity of antibodies to target a single antigen
B) directly examine the organism's appearance or behavior, which includes its metabolic abilities, environmental preferences and drug susceptibilities
C) analyze the genetic makeup of the microorganism, which conclusively diagnoses the infection
D) amplifies the microbial DNA in the patient's sample and during the process, identifies the organism through the use of known primers

E) A) and B)
F) C) and D)

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In vivo testing is useful for diagnosing ________.


A) tuberculosis
B) rubella virus
C) hepatitis A
D) HIV
E) whooping cough

F) B) and C)
G) A) and B)

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Which sample is not typically collected by sterile needle aspiration?


A) Blood
B) Urine
C) Cerebrospinal fluid
D) Tissue fluids
E) All of the choices are collected by sterile needle aspiration.

F) A) and B)
G) B) and E)

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Animals are required for the cultivation of ________.


A) Treponema pallidum
B) Pseudomonas
C) Streptococcus
D) Salmonella
E) Clostridium

F) C) and E)
G) A) and D)

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A

Antibody testing requires ________.


A) a known antigen
B) a known antibody
C) both a known antigen and a known antibody
D) either a known antigen or a known antibody

E) B) and C)
F) None of the above

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Which test is especially good for bacteria that are not readily cultivated in the lab?


A) Gram stain
B) Direct antigen testing
C) Dichotomous key
D) Direct fluorescence antibody (DFA) testing
E) Phage test

F) None of the above
G) B) and E)

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Analysis of the laboratory samples yield a diagnosis of herpes simplex virus. The patient's mother is upset, as she does not believe her son is sexually active. The nurse explains that the presence of HSV does not imply sexual contact and provides education about the accuracy of the results. Which of the following is the best rationale for confidence in the diagnosis?


A) Signs and symptoms are diagnostic.
B) PCR tests are highly sensitive and specific.
C) The Gram stain is an excellent screen for HSV.
D) HSV can be definitively identified by light microscopy.

E) C) and D)
F) All of the above

Correct Answer

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A positive serological test for tuberculosis indicates that ________.


A) the patient has active tuberculosis
B) the patient is an asymptomatic carrier of tuberculosis
C) the patient has been exposed to tuberculosis
D) All of the choices are correct.

E) A) and B)
F) B) and D)

Correct Answer

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When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

A) True
B) False

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